Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4

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Identification of the Inducing Agent of the 2,4-Dichlorophenoxyacetic Acid Pathway Encoded by Plasmid pJP4.

The inducing agent of the 2,4-dichlorophenoxyacetic acid (2,4-D) pathway of Alcaligenes eutrophus JMP134 (pJP4) was determined through the analysis of promoterless lacZ transcriptional fusions with tfd structural genes. (beta)-Galactosidase activity was measured in the presence and absence of 2,4-D. Fusions of the individual genes act both as reporters and disrupters of gene expression. Increas...

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Comparison of 2,4-dichlorophenoxyacetic acid degradation and plasmid transfer in soil resulting from bioaugmentation with two different pJP4 donors.

A pilot field study was conducted to assess the impact of bioaugmentation with two plasmid pJP4-bearing microorganisms: the natural host, Ralstonia eutropha JMP134, and a laboratory-generated strain amenable to donor counterselection, Escherichia coli D11. The R. eutropha strain contained chromosomal genes necessary for mineralization of 2,4-dichlorophenoxyacetic acid (2,4-D), while the E. coli...

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phylogeography and genetic diversity of the lesser mouse- eared bat (myotis blythii) in iran

in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...

Characterization of diverse 2,4-dichlorophenoxyacetic acid-degradative plasmids isolated from soil by complementation.

The diversity of 2,4-dichlorophenoxyacetic acid (2,4-D)-degradative plasmids in the microbial community of an agricultural soil was examined by complementation. This technique involved mixing a suitable Alcaligenes eutrophus (Rifr) recipient strain with the indigenous microbial populations extracted from soil. After incubation of this mixture, Rifr recipient strains which grow with 2,4-D as the...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1985

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.161.1.466-468.1985